Composite
Part:BBa_K3002210:Design
Designed by: Dorothée Klein Group: iGEM19_TU_Kaiserslautern (2019-10-21)
L2 spectinomycin resistance + ARS_Mut-PETase + cCA_MHETase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5303
Illegal PstI site found at 3491
Illegal PstI site found at 4464
Illegal PstI site found at 6645
Illegal PstI site found at 6969
Illegal PstI site found at 7312
Illegal PstI site found at 8122 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5303
Illegal NheI site found at 2665
Illegal NheI site found at 5567
Illegal PstI site found at 3491
Illegal PstI site found at 4464
Illegal PstI site found at 6645
Illegal PstI site found at 6969
Illegal PstI site found at 7312
Illegal PstI site found at 8122
Illegal NotI site found at 6980 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5303
Illegal BglII site found at 7890 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5303
Illegal PstI site found at 3491
Illegal PstI site found at 4464
Illegal PstI site found at 6645
Illegal PstI site found at 6969
Illegal PstI site found at 7312
Illegal PstI site found at 8122 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5303
Illegal PstI site found at 3491
Illegal PstI site found at 4464
Illegal PstI site found at 6645
Illegal PstI site found at 6969
Illegal PstI site found at 7312
Illegal PstI site found at 8122
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3226
Illegal NgoMIV site found at 3253
Illegal NgoMIV site found at 4904
Illegal NgoMIV site found at 6578 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
This part is a combination of the parts BBa_K3002102 (spectinomycin resistance), BBa_K3002108 (ARS_Mutant PETase) and BBa_K3002113 (cCA_wildtype MHETase).